Differentiated DCs from transduced human cells that had engrafted in the NOD/SCID mice.

Human cells derived from transduced CB CD34⁺ cells were isolated from the BM of engrafted NOD/SCID mice (Figure 3C). Enriched human cells were cultured with human GM-CSF, IL-4, and TNF-α for 14 days to allow DC differentiation and maturation as in Figure 1. (A) FACS analysis of GFP and other DC surface marker (including MHC II) expression in differentiated human DCs after transplantation. The control group shows the differentiated DCs without GFP transduction. (B) Quantification of the transgene expression specificity of either vector in MHC II⁺ cells among DC- and CFC-derived progeny (non-DCs, as in Figure 2C) differentiated in vitro subsequent to the engraftment of transduced CD34⁺ cells.