Fig. 4.
![Fig. 4. ET-1 binding in normal and sickle erythrocytes. / Specific binding of [125I]–ET-1 as a function of radiolabeled ET-1 concentration. Scatchard plot of the specific binding of iodinated ET-1 gave a Kd of 244 ± 105 nM and 336 ± 78 sites/per cell for sickle red cells. TheKd for normal red cells was 128 ± 73 nM, with a maximal binding capacity of 238 ± 53 sites/cell. No statistically significance differences were found between sickle and normal erythrocytes. The line represents the nonlinear regression of experimental points. Values are mean ± SE of 3 similar experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/1/10.1182_blood.v99.1.357/6/h80121967004.jpeg?Expires=1724239320&Signature=m2bVEO2GuJQvLfG-J~L63OF2CKhhO94bcdigWNVMDfOCNr~RXL-b9GJd5CvQs1AudDgRsj5aC2eWlqXbOw5HoBdqGqu8Z3S6gADm6ARK4sBDsdUBH9J-YB3NspaTTd~9OAFTKpMktYybvTg5MdJ0tRHNXPwYwRrhjI0wZsxuUKE1KwXurGXtJrtzLHD-VoS0xfJASTGFpYd3da9WWDldnDqo~2hFO1XikLHh6n4hbqQiS71OmHVbVtR27mMQkZzC~vbVYX2j-txFeB8KlDETOWj2N8fz96wBwMBMtMVUwy0RM9vHr3Nb8xCmBsNDZUKtAlx~M24DYuaZQ8WD9oyjnw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
ET-1 binding in normal and sickle erythrocytes.
Specific binding of [125I]–ET-1 as a function of radiolabeled ET-1 concentration. Scatchard plot of the specific binding of iodinated ET-1 gave a Kd of 244 ± 105 nM and 336 ± 78 sites/per cell for sickle red cells. TheKd for normal red cells was 128 ± 73 nM, with a maximal binding capacity of 238 ± 53 sites/cell. No statistically significance differences were found between sickle and normal erythrocytes. The line represents the nonlinear regression of experimental points. Values are mean ± SE of 3 similar experiments.
