Effects of cytokines on the Ca⁺⁺-activated K⁺ influx (Gardos channel) in normal and Duffy-negative erythrocytes.

Channel activity was expressed as the difference between the total K⁺ influx in the presence of A23187 ionophore (1 μM ionized Ca⁺⁺). The K⁺ influx was measured in the presence and the absence of 50 nM ChTX. Influx media contained the indicated amount for each of these agents: 20 ng/mL (370 nM) IL-10; 20 ng/mL (2.6 nM) RANTES; 100 nM PAF; 500 M ET-1. Results are expressed as the mean ± SE of 3 experiments in duplicate determinations. The statistical difference was determined using the control values against the values in the presence of the cytokines for each cell type. **P < .03; *P < .05.