Fig. 4.
Fig. 4. Analysis of PCR products for TCR-γ chain gene on polyacrylamide gels in AITL. / (Top panel) All cases demonstrated in the figure show 1 or 2 dominant bands consistent with a monoclonal T-cell population. Lane M, molecular weight markers; lane +, positive control; lane 1, case 21; lane 2, case 23; lane 3, case 26; lane 4, case 22; lane 5, case 3; lane 6, case 4; and lane −, negative control. (Bottom panel) Microdissection of CD10+ cells in cases 26 and 13 gave PCR products identical in size to products obtained from whole-section PCR. Lane 1, whole-section PCR products of case 26; lanes 1a,b,c, microdissected PCR products of case 26; lane 2, whole section PCR products of case 13; lanes 2a,b,c, microdissected PCR products of case 13; and lane −, negative control.

Analysis of PCR products for TCR-γ chain gene on polyacrylamide gels in AITL.

(Top panel) All cases demonstrated in the figure show 1 or 2 dominant bands consistent with a monoclonal T-cell population. Lane M, molecular weight markers; lane +, positive control; lane 1, case 21; lane 2, case 23; lane 3, case 26; lane 4, case 22; lane 5, case 3; lane 6, case 4; and lane −, negative control. (Bottom panel) Microdissection of CD10+ cells in cases 26 and 13 gave PCR products identical in size to products obtained from whole-section PCR. Lane 1, whole-section PCR products of case 26; lanes 1a,b,c, microdissected PCR products of case 26; lane 2, whole section PCR products of case 13; lanes 2a,b,c, microdissected PCR products of case 13; and lane −, negative control.

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