Increases in SDF-1, CFCs, and WBCs in blood after FucS treatment.

Plasma levels of SDF-1α and SDF-1β (heavy lines), total WBCs (open circle and dashed line), and CFCs (bars) were measured in untreated BDF1 mice or after treatment with FucS. (A) Mice were intravenously injected with 50 or 100 mg/kg FucS and bled at 3 hours. Differences were statistically significant by the Student t test between groups treated with 50 or 100 mg/kg (n = 5 per group, CFCs and WBCs,P < .01; SDF-1α, P < .005; SDF-1β,P < .001). (B) Mice were bled 3 hours after the last of 1 or 3 intravenous injections of 100 mg/kg FucS at 1 injection per day. Differences were statistically significant between groups treated once or thrice (n = 5 per group, SDF-1α, P < .02; CFCs,P < .000 01; WBCs and SDF-1β,P < .0001). All treated to untreated (n = 6) values were significant (P < .000 01) in panels A and B. (C) Untreated mice (0 hours) and mice intravenously injected once with 100 mg/kg FucS were bled at the indicated times. All treated to untreated values were significant statistically (n = 5 per group;P < .0001). Error bars represent the mean ± SEM.