Generation of DCs.

(A) Flow cytometric analysis on splenic cells of Balb/cRAG2^−/−(H-2^d) mice that received transplantations 3 months earlier of Pax5^−/−H-2^b/d pre-B I cells. (B) Mixed lymphocyte reaction. CD11c⁺CD8α⁺ and CD8α⁻ cells ofPax5^−/−origin (H-2^b) and of host Balb/c RAG2^−/−(H-2^d) were sorted and γ-irradiated, and 3 × 10³ of these were incubated for 5 days with 2 × 10⁵ Balb/c or C57Bl/6 lymph node cells. γ-Irradiated spleen cells (1 × 10⁶) of Balb/c or C57Bl/6 mice were used as controls. [³H]-thymidine incorporation was measured during the last 14 hours of incubation. Stimulators were CD11⁺CD8α⁺H-2^b/d, CD11⁺CD8α⁺H-2^d, CD11⁺CD8α⁻H-2^b/d, CD11⁺CD8α⁻H-2^d, and Balb/c H-2^d splenocytes, and C57Bl/6 H-2^b splenocytes.