Representative autoradiograph of VWCP activity assay.

Undigested VWF and VWCP-proteolyzed VWF were detected by antibody labeling and radiography as described in “Patients, materials, and methods.” Horizontal lines indicate the 15th and 85th percentiles of the undigested VWF multimer range. Lanes 1 and 2, undigested VWF; lane 3, normal VWCP activity; lanes 4, 7, and 8, intermediate VWCP activity; lanes 5 and 6, deficient VWCP activity.