Clustering of membrane rafts and Fas in ET-18-OCH₃–treated Jurkat cells.

(A) Time course of the effect of ET-18-OCH₃ on aggregation of membrane rafts. T-leukemic Jurkat cells, grown in 10% FCS-containing medium, were either untreated (Control) or treated with 5 μg/mL ET-18-OCH₃ for the times indicated. Cells were then stained with FITC-CTx and analyzed by confocal microscopy. Bar indicates 7 μm. (B) Colocalization of membrane rafts (Raft) and Fas in ET-18-OCH₃–treated Jurkat cells. Cells were either untreated (Control) or treated with 5 μg/mL ET-18-OCH₃for 3 hours, and processed for confocal microscopy using FITC-CTx (green fluorescence for lipid rafts) and anti-Fas mAb, followed by CY3-conjugated antimouse antibody (red fluorescence for Fas). Areas of colocalization between membrane rafts and Fas in the overlay panels are yellow. Bar indicates 10 μm.