Effect of rhTIMP-1 on survival after radiation of Hodgkin-derived cell lines (FACS analysis).

(A) FACS analysis of apoptosis in the L591 cell line; shown are baseline levels (i), levels of radiation-induced apoptosis or cell death (ii), and levels of radiation-induced apoptosis or cell death with rhTIMP-1 present (iii). LL indicates viable cells with little PI and annexin V staining; LR, annexin V–positive cells; and UR, annexin V–positive and PI-positive cells. (B) Results for all cell lines tested. R− indicates no irradiation (left column); R+, after irradiation; Co, control cells irradiated in the absence of rhTIMP-1; TIMP-1, cells irradiated with rhTIMP-1 present; asterisk, viable cells expressed as the percentage with no annexin V or PI staining (ie, LL cells on FACS analysis).