Fig. 6.
Fig. 6. Levels of TNF expression incux/CDPΔHD/ΔHD tissues are elevated. / (A) Cells from 6 pairs of control andcux/CDPΔHD/ΔHD bone marrow, and ConA- stimulated spleen and thymus were cultured for 24 and 72 hours, after which supernatants were harvested and analyzed for levels of TNF by ELISA. Levels of secreted TNF fromcux/CDPΔHD/ΔHD cells relative to cells from control littermates are depicted. (B) Levels of TNF in tissue extracts from 8 pairs of mice (n = 3 for bone marrow) were analyzed for TNF protein by ELISA. Mean levels are indicated with bars. Asterisks indicate directional Wilcoxon signed-rank tests of statistical significance P less than .05.

Levels of TNF expression incux/CDPΔHD/ΔHD tissues are elevated.

(A) Cells from 6 pairs of control andcux/CDPΔHD/ΔHD bone marrow, and ConA- stimulated spleen and thymus were cultured for 24 and 72 hours, after which supernatants were harvested and analyzed for levels of TNF by ELISA. Levels of secreted TNF fromcux/CDPΔHD/ΔHD cells relative to cells from control littermates are depicted. (B) Levels of TNF in tissue extracts from 8 pairs of mice (n = 3 for bone marrow) were analyzed for TNF protein by ELISA. Mean levels are indicated with bars. Asterisks indicate directional Wilcoxon signed-rank tests of statistical significance P less than .05.

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