Fig. 10.
PGPC is one biologically active component in OxPAPC.
(A) PLA2 or mock-treated OxPAPC was separated by TLC into phospholipids (PL) and fatty acids (FA). The fractions were eluted, evaporated, and used for stimulation of HUVECs. The lipid fractions were added in amounts equivalent to 150 μg/mL OxPAPC. After 6 hours, the cells were washed and analyzed in duplicate for TF activity by clotting assay. (B) HUVECs were stimulated with indicated concentrations of POVPC or PGPC for 6 hours. At the end of incubation, the cells were analyzed for TF activity. Similar results were obtained in 2 independent experiments.
