ERK1/2 and EGR-1 are important for OxPAPC-induced TF expression.

(A) HUVECs were pretreated for 20 minutes with indicated concentrations of the MEK inhibitor PD98059 and then stimulated with 125 μg/mL OxPAPC for 6 hours in the presence of the inhibitor. Cells were harvested and TF activity was determined by clotting assay. Data are expressed as percentage of activity in cells treated with vehicle (0.1% DMSO) only and are representative for 2 independent experiments. DMSO (0.1% final concentration) alone had no effect. (B) HUVECs were infected with either control (AdGFP) or NAB2-adenovirus (AdNAB2), and 24 hours after infection stimulated with OxPAPC (125 μg/mL) for 6 hours. After stimulation, TF activity was determined by clotting assay. Data are presented as means ± SD of 3 measurements. Similar results were obtained in another independent experiment.