Reproductive phenotype of

Csf1r⁻/Csf1r⁻mice. (A) Duration of estrus cycle in virgin female mice (5 mice per genotype, 8 cycles/mouse, ± SD). (B) Percentage time in proestrus (P), in estrus (E), in metestrus (M), and in diestrus (D) (± SD). (C) Whole-mount alum-carmine staining of the 4th inguinal mammary gland from 18-day pregnant mice. All panels are at the same magnification, approximately only one third of the wild-type gland is shown. LN indicates lymph node. (D) Percentage of successful pregnancies resulting from the consecutive daily mating of wild-type (open) andCsf1r⁻/Csf1r⁻(filled) male mice with superovulated virgin female mice. *Indicates significantly different from wild type; wt, wild type.