Effect of upstream intron mutations on exon 16 splicing in induced MEL cells.

In construct GI, as well as in GI(a), GI(b), GI(b,c), and GI(a,c), globin intronic fragments (open boxes) were substituted for 4.1R sequences. Data are expressed as percentage of exon 16 inclusion in both uninduced and induced cells. These percentages were averaged from 3 independent RT-PCR experiments ± SD.