Prelymphoid origin of G8600A mutation in B-CLL 33.

(A) G8600A mutation in DNA from tumor cells of patient with B-CLL 33 (i), separated to 99.8% purity using CD19 PE-coated magnetic beads (ii). (B) G8600A mutation together with a small peak of a normal sequence in DNA from CD3⁺ cells of patient with B-CLL 33 (i), separated to 95% purity using CD3 PE-coated magnetic beads (ii). (C) G8600A mutation in DNA from CD14⁺ cells of patient with B-CLL 33 (i) separated to 100% purity using CD14 labeling and FACS sorting (ii). (D) Absence of G8600A mutation in patient's buccal epithelial cells.