Anion transport studies of band 3 Walton.
(A) DIDS titration of sulfate influx into erythrocytes. The number of DIDS binding sites was determined by titration of the influx of [35S]sulfate into erythrocytes, as described.5,18 Control erythrocytes (▾); B1 erythrocytes (○); B2 erythrocytes (●). Three replicate measurements were taken for each point. The results show the mean and the error bars the SD of the 3 replicate measurements. (B) Chloride influx intoXenopus oocytes. Oocytes were injected with 1.5 ng kidney band 3 cRNA (K) or kidney band 3 Walton cRNA (KW) with or without 0.15 ng glycophorin A cRNA (A). After 24 hours, Cl− influx over 1 hour was measured individually on 13 to 15 oocytes, in the presence or absence of 2 mM 4,4′-dinitrostilbene-2,2′-disulfonate. Control oocytes were injected with water. The results show the mean stilbene disulfonate–sensitive Cl− influx for each cRNA, and the error bars indicate the SEM for each sample.
