Role of cytoskeletal reorganization and intracellular calcium in regulating tether dynamics.

Platelets were pretreated with vehicle alone (control), cytochalasin D (CD) (5 μM), or DM-BAPTA (70 μM) prior to perfusion through VWF-coated microcapillary tubes at 150 s⁻¹ for 5 seconds. The wall shear rate was either maintained at 150 s⁻¹ or increased to 600 s⁻¹, 1800 s⁻¹, 5000 s⁻¹, or 10 000 s⁻¹. Translocating platelets from 10 random fields were video-recorded for analysis of tether length (A) and lifetime (B). These results are from 3 independent experiments (P < .1; P < .05;P < .001).