American Society of Hematology

Fig. 3.

$Fig. 3. Specificity of antibodies against JAM family members. / (A) Specificity of antibodies directed against JAM-1, JAM-2, and JAM-3. MDCK cells stably transfected with cDNA encoding murine JAM-1, JAM-2, or FLAG-tagged JAM-3 were stained, as indicated with, antibodies to JAM-1 (H202-106), JAM-2 (XVIIIF26), JAM-3 (polyclonal antibody), or FLAG peptide (M2). Dashed profiles represent negative controls obtained by omitting the primary antibody. (B) Expression of JAM members by KLN205 epithelial cells. Cells were stained with monoclonal antibodies against JAM-1 (i), JAM-2 (ii), or JAM-3 (iii) as indicated. Negative control (iv) was obtained by using an IgG fraction from nonimmune rabbit serum. Secondary antibodies were antirat and antirabbit coupled to FITC. Magnification × 630.$

Specificity of antibodies against JAM family members.

(A) Specificity of antibodies directed against JAM-1, JAM-2, and JAM-3. MDCK cells stably transfected with cDNA encoding murine JAM-1, JAM-2, or FLAG-tagged JAM-3 were stained, as indicated with, antibodies to JAM-1 (H202-106), JAM-2 (XVIIIF26), JAM-3 (polyclonal antibody), or FLAG peptide (M2). Dashed profiles represent negative controls obtained by omitting the primary antibody. (B) Expression of JAM members by KLN205 epithelial cells. Cells were stained with monoclonal antibodies against JAM-1 (i), JAM-2 (ii), or JAM-3 (iii) as indicated. Negative control (iv) was obtained by using an IgG fraction from nonimmune rabbit serum. Secondary antibodies were antirat and antirabbit coupled to FITC. Magnification × 630.

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