Relative expression of transcripts encoding JAM family members.

(A) Northern blot analysis of JAM-3 (i), JAM-1 (ii), or JΑΜ-2 (iii) transcripts on polyA mRNA extracted from indicated tissues. JAM-3 probe (i) gives 2 hybridization signals at ≈1.5 and 4.4 kb, whereas JAM-1 (ii) and JAM-2 (iii) probes give a single hybridization signal at ≈2 kb. (B) Results of reverse PCR analysis for JAM-1 (i), JAM-2 (ii), JAM-3 (iii), or HPRT (iv) on cDNA from indicated cell lines. Positive controls (Ctrls) were obtained by running the PCR with plasmids encoding full-length sequences of JAM-1, -2, and -3. Negative controls were obtained by omitting the reverse transcription step, resulting in an absence of detectable signals. MLN indicates mesenteric lymph nodes.