JAM subfamily sequences and phylogenetic trees.
(A) Multiple alignment of murine JAM-1, JAM-2, and JAM-3 amino acid sequences. Identical and homologous amino acids are shaded in dark and light gray, respectively. The β-strand–loop immunoglobulin domain organization is indicated, and the letters refer to the strands of the V and C2 domains. Murine sequences for JAM-1 and JAM-2 are accessible from the database under the respective accession numbers, U89915 and AJ300304. JAM-3 has been submitted to EMBL Nucleotide Sequence Database and is accessible under the numberAJ291757. (B) Phylogenetic trees obtained with the Clustal X program using the neighbor-joining method. Positions with gaps were excluded, and bootstrap values are indicated at the knots and phylogenetic distances over the branches. Grouping of the JAM subfamily is obtained by comparison of the V+C extracellular domains, andDrosophila amalgam is used as an outgroup. The same grouping was obtained using full-length sequences.
