Phosphoamino acid analysis of FANCA.

FANCA protein phosphorylated in vivo (A) and in vitro (B) were subjected to phosphoamino acid analysis. As a control of threonine phosphorylation, GST-CaM-KI(1-293, Lys49Glu), phosphorylated by GST-CaM-KK(84-434) in vitro, was subjected to phosphoamino acid analysis (C). The positions of phosphoamino acid standards, as determined by ninhydrin staining, are shown.