In situ phosphopeptide mapping of FANCA.

Flag-tagged wt-FANCA expressed in GM6914 cells was phosphorylated in vivo or immunoprecipitated with either anti-FANCA antibody or anti-Flag monoclonal antibody and phosphorylated in vitro. Phosphorylated FANCA proteins were treated with V8 protease (0.5 or 5 μg) and separated on an SDS-polyacrylamide gel.