FITC-LPS binding to monocytes in the presence of healthy control sera, rhLBP-supplemented control sera, and LBP-depleted severe sepsis sera.

(A) Mean fluorescence of monocytes incubated with FITC-LPS in the presence of control sera and control sera supplemented with 100 μg/mL rhLBP. Data are presented as 25th (box), 50th (median), and 75th percentiles (box) and range (-). Statistical analysis was performed with the Mann-Whitney U test; *P < .05. (B) Mean fluorescence of monocytes incubated with FITC-LPS in the presence of control sera supplemented with increasing concentrations of rhLBP assessed by FACS analysis. Data are presented as mean ± SEM of 5 independent experiments. (C) Patient example of the FITC-LPS FACS experiment: mean fluorescence of monocytes incubated with FITC-LPS in the presence of severe sepsis serum (dark gray graph) and partially LBP-depleted severe sepsis serum (light gray graph).