FITC-LPS binding to monocytes in the presence of severe sepsis sera and healthy control sera.

(A) Mean fluorescence of monocytes incubated with FITC-LPS in the presence of 15% serum from patients with severe sepsis or from healthy controls. Data are presented as 25th (box), 50th (median), and 75th percentiles (box) and range (-). Statistical analysis was performed with the Mann-Whitney U test; *P < .05. (B) Patient example of the FITC-LPS FACS experiment: Monocytes were incubated with FITC-LPS in the presence of 15% serum from a healthy volunteer (light gray graph) and serum from a randomly chosen patient with severe sepsis (dark gray graph).