Fig. 6.
Effect of P2 receptor antagonists on LIBS6-induced thromboxane A2 generation.
Platelets were activated with Fab fragments of the LIBS6 antibody (1.5 μM) at 37°C with stirring for 5 minutes. P2 receptor subtype–selective antagonist (1 μM AR-C67085 or 1 mM A2P5P) was added, as indicated, just prior to the addition of fibrinogen (3 μM), and stirring was continued for an additional 3 minutes. The reaction was stopped by snap-freezing, and thromboxane B2 was measured as described in Figure 1. The data were normalized to thromboxane B2 generated by LIBS6 and fibrinogen, taken as 100%. The results were from 2 independent experiments performed in duplicate.
