![Fig. 1. H90 and A3D8 anti-CD44 mAbs inhibit proliferation of HL60, NB4, THP-1, and KG1a cell lines. / A total of 105 cells/mL were seeded into 96-well plates and incubated in triplicate in the presence of specific anti-CD44 mAbs (2.5 μg/mL A3D8 or 20 μg/mL H90). Controls were cells incubated with isotypic control (20 μg/mL IgG1). At the indicated times, the viable cell number was determined using trypan blue dye exclusion (A). The statistical difference (P < .05) between A3D8-treated cells and controls is reached from day 2 (P < .05). The one between H90-treated cells and controls is reached from day 3 in HL60, NB4, and THP-1 cells and day 2 in KG1a cells. Treatment with the anti-CD44 mAb J173, which is inactive, gives similar results as the isotypic control. ▪: control; ▴: + H90; ■: +A3D8. At day 3 (exponential growth of control cells), [3H]thymidine incorporation into the cellular DNA was measured (B). Data are means ± 1 SD of 3 independent experiments. Data obtained with A3D8- or H90-treated cells are significantly different from those of controls (P < .05). Light grey bar: control; medium grey bar: +A3D8; black bar: + H90.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/1/10.1182_blood.v99.1.290/6/h80121969001.jpeg?Expires=1724232869&Signature=gWAi2bVHgFLSwmWfF9eQs4YMAtsx4KQTpHzYKdRV9005W3hcJ2wZSbDa~pDhlhei2I5JOtpkG-2vBl2ceg-bOYexNvr~woi4jHcbQ3Jtq2HY-C1o0UXulEkQwxDG6Ml2MSgY7u5YDbGxTZf5PvSY5JUiBMQJboLl2rNp4B~Cpft5u8DNKN6hom-sOgFVw719tpj3yYIyb7P4KEzwQQwIlOcBuytn0AFaPz7ds96cR4fnMXR9Zik0UpFuh1rOhaX4JQtrCUxMClYUqxd7zPOSHLbhQB5LraAO5DdaehN06r7sTLEcGIgAnqomEzVjKl0TIb-tOM0Zi548leLRFOpU6g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
H90 and A3D8 anti-CD44 mAbs inhibit proliferation of HL60, NB4, THP-1, and KG1a cell lines.
A total of 105 cells/mL were seeded into 96-well plates and incubated in triplicate in the presence of specific anti-CD44 mAbs (2.5 μg/mL A3D8 or 20 μg/mL H90). Controls were cells incubated with isotypic control (20 μg/mL IgG1). At the indicated times, the viable cell number was determined using trypan blue dye exclusion (A). The statistical difference (P < .05) between A3D8-treated cells and controls is reached from day 2 (P < .05). The one between H90-treated cells and controls is reached from day 3 in HL60, NB4, and THP-1 cells and day 2 in KG1a cells. Treatment with the anti-CD44 mAb J173, which is inactive, gives similar results as the isotypic control. ▪: control; ▴: + H90; ■: +A3D8. At day 3 (exponential growth of control cells), [3H]thymidine incorporation into the cellular DNA was measured (B). Data are means ± 1 SD of 3 independent experiments. Data obtained with A3D8- or H90-treated cells are significantly different from those of controls (P < .05). Light grey bar: control; medium grey bar: +A3D8; black bar: + H90.
