Tyr432 (Y4) on human EPOR is important for

c-myc gene induction. UT-7/EPO cells having series of chimeric receptors were starved for 24 hours and then exposed to EPO (10 U/mL) or G-CSF (250 ng/mL). One hour later, total cellular RNA was isolated and subjected to Northern blot analysis for c-myc mRNA expression. Ethidium bromide staining of the gels is shown as a loading control.