Growth curve of the transfectant cells expressing chimeric receptors in response to G-CSF.

Parental UT-7/EPO cells or the transfectants expressing GEY0, GEY3, GEY5, GEY8, GEY4F, or GEY5F were cultured with various concentrations of G-CSF (0.25 to 2500 ng/mL) or EPO (10 U/mL). MTT reduction assay was performed after 3 days of culture. The values represent the mean ± SD from triplicate cultures and are expressed as a percentage of maximal response to EPO.