Flow cytometric analysis of expression of chimeric receptors on the cell surface of UT-7/EPO–derived transfectants.

Parental UT-7/EPO cells and each transfectant were incubated with mouse antihuman G-CSFR antibodies. Then the cells were stained with fluorescein-conjugated rabbit antimouse antibody and analyzed by flow cytometry. The thin line in each panel indicates the expression profile of parental UT-7/EPO cells as a negative control. The thick line in each panel indicates the expression profile of each transfectant.