CD2 LCR partially shields transplanted cells by silencing in vivo.

The human T lymphoid cell line Molt-3 was transduced with the LESN or the LESNΔ-LCR retroviral vectors generated by transient transfection of 293T cells and was selected in G418-containing medium for 4 weeks. At that time, the percentage of HLA class I⁺and of EGFP-expressing cells was quantified by FACS analysis (in vitro panels). Subsequently, vector-transduced cells were subcutaneously implanted in SCID mice and were grown for 4 weeks before recovery and measurement of HLA-I and EGFP expression (ex vivo panels). The percentage of positive cells for each marker, MFI, and CV of the analyzed cell population are indicated.