Transduction of human lymphoid Jurkat and murine fibroblastic NIH-3T3 cells with different retroviral vectors carrying the CD2 LCR shows that modulation of EGFP expression is tissue specific.

Cells were infected with LESN, LESNΔ, LESNΔ-LCR, and LESNΔ-NLCR retroviral vectors generated by transient transfection of 293T cells. Four weeks after transduction and selection in G418-containing medium, the percentage of EGFP-expressing cells was quantified by FACS analysis. The percentage of EGFP⁺ cells for each construct, MFI, and CV are also reported in each panel. Profile of the mock-transduced lymphoid cells is shown as a continuous line.