Transduction of the human T-lymphoid Molt-3 cells with different retroviral vectors carrying the CD2 LCR and a deletion in the viral enhancer leads to a homogeneous, unimodal pattern of EGFP expression.

(A) Molt-3 cells were infected with LESN, LESNΔ, LESNΔ-LCR, and LESNΔ-NLCR retroviral vectors generated by transient transfection of 293T cells and were selected in G418-containing medium for 4 weeks before FACS analysis. Representative patterns of EGFP expression are shown. The percentage of EGFP⁺ cells for each construct, MFI, and CV are indicated. The profile of the mock-transduced lymphoid cells in each panel is shown as a continuous line. (B) Representative pattern of EGFP expression in Molt-3 cells transduced with the vectors indicated above each panel. Same cell populations analyzed by FACS and shown in panel A.