The effect of 5T2MM cells on the development of bone disease in C57BL/KaLwRijHsd mice.

(A) Histologic section of the tibia from a control animal showing cortical bone and the presence of normal marrow. Original magnification × 20. (B) Histologic section of the tibia from a 5T2MM-bearing animal showing cortical bone and demonstrating the complete replacement of normal marrow by 5T2MM cells. Original magnification × 20. (C) Section reacted for TRAP activity showing the presence of 5T2MM cells found closely associated with TRAP-positive osteoclasts (arrows) lining the bone surface. Original magnification × 40. (D) Section of the tibia from an animal injected with 5T2MM cells showing resorption through the cortical bone. Original magnification × 10. (E) Radiograph of the tibia of an animal bearing 5T2MM cells showing the presence of lytic bone lesions (arrows).