TGF-β1 and TβR II expression in different subsets of single human hematopoietic cells.

Flow cytometry–sorted cells were individually isolated by micromanipulator and analyzed using poly-dT–primed RT-PCR probed with radiolabeled gene-specific cDNA. Panels of 5 cells of each type were used. Quantitative analysis of the tested genes normalized to GAPDH signals with densitometry software (NIHimager) are presented in the table.