Ac-DEVD-CHO, a caspase-3–specific inhibitor, can prevent As₂O₃-induced apoptosis.

HUT-102 cells were preincubated with Ac-DEVD-CHO at 100 μM, for 2 hours, then treated with control buffer or with As₂O₃ + IFN-α for 60 hours in the presence of Ac-DEVD-CHO. XTT assay was then performed, as described in “Materials and methods.” Graph represents the mean of 2 separate experiments performed in triplicate. Lane 1, control treatment; lane 2, As₂O₃ + IFN-α; lane 3, As₂O₃ + IFN-α + Ac-DEVD-CHO.