Fig. 5.
Fig. 5. As2O3 induces caspase-3 activation and Bcl-2 cleavage. / Sixty micrograms total proteins from MT-2 cells were resolved on 4% to 20% Tris-glycine gels transferred to PVDF membranes and probed with specific antibodies for (A) caspase-3 and (B) PARP. Comparable protein loading was verified using an antibody specific for the housekeeping gene product β-tubulin (data not shown). (C) Sixty micrograms mitochondrial-enriched fraction proteins from MT-2 or HUT-102 cells was resolved on 4% to 20% Tris-glycine transferred to PVDF membranes and probed with specific antibodies for Bcl-2. (A-C) Results are representative of at least 2 independent experiments using different HTLV-1–infected cells (B-C, panel 1). Lane 1, control treatment; lane 2, IFN-α; lane 3, As2O3; lane 4, As2O3 + IFN-α. (A, C, panels 2 and 3) Lane 1, control treatment; lane 2, As2O3+ IFN-α.

As2O3 induces caspase-3 activation and Bcl-2 cleavage.

Sixty micrograms total proteins from MT-2 cells were resolved on 4% to 20% Tris-glycine gels transferred to PVDF membranes and probed with specific antibodies for (A) caspase-3 and (B) PARP. Comparable protein loading was verified using an antibody specific for the housekeeping gene product β-tubulin (data not shown). (C) Sixty micrograms mitochondrial-enriched fraction proteins from MT-2 or HUT-102 cells was resolved on 4% to 20% Tris-glycine transferred to PVDF membranes and probed with specific antibodies for Bcl-2. (A-C) Results are representative of at least 2 independent experiments using different HTLV-1–infected cells (B-C, panel 1). Lane 1, control treatment; lane 2, IFN-α; lane 3, As2O3; lane 4, As2O3 + IFN-α. (A, C, panels 2 and 3) Lane 1, control treatment; lane 2, As2O3+ IFN-α.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal