Hierarchical inhibition by AF of IFN-γ and IL-2 production in peripheral blood T cells.

Peripheral blood T cells were stimulated in the presence of fixed activated monocytes, with increasing PHA concentrations, with or without AF or PP14-depleted AF at the indicated concentrations. Conditioned media were collected 48 hours later, and IL-2 (upper panel) and IFN-γ (lower panel) secretions were determined by ELISA. Comparable results were obtained in 4 separate experiments.