AML1-ETO expression in human HPCs preserves an immature myeloid phenotype.

Over a 5-week culture on MS-5 stroma, the nonadherent cells were weekly counted by trypan blue, stained for CD34 and CD14 expression, and analyzed by flow cytometry. (A) CD34 and CD14 expression of cells present at week 4. (B) Quantitation of CD34⁺ cells present at weekly intervals over a 4-week period. (C) Wright-Giemsa stain of the nonadherent cells after 5 weeks in MS-5 stromal culture, showing macrophage morphology in the MIGR1-transduced cultures and an immature myeloid phenotype in the MIGR1-AE sample. Magnification is 1000 ×.