Transduction of human HPCs and expression of AML1-ETO.

(A) The MIGR1 retroviral vector is shown schematically. AML1-ETO cDNA was subcloned into the multiple cloning site located upstream of the internal ribosome entry site (IRES) element. (B) A representative flow cytometry assay to assess the efficiency of retroviral transduction of human HPCs, showing the percentage of transduced cells calculated on the basis of GFP expression. (C) Western blot analysis demonstrating expression of the correct-size AML1-ETO protein in transduced human HPCs. The Kasumi cell lysate was loaded at 3 different concentrations to allow comparison with the level of AML1-ETO expression in the transduced CD34⁺cells.