Fig. 9.
Effect of anti–IL-8 antibodies on PMN migration across HUVECs induced by iTCC.
Cells were incubated with iTCC (5 nM), TNF-α (1.5 × 103U/mL), or IL-8 (10−8 M) either alone (░) or mixed with affinity-purified rabbit antibodies to IL-8 (10 μg/mL) (▪). PMNs were introduced into the upper chamber, and the percentage of migrated PMNs was evaluated after an incubation of 30 minutes. Values are means ± SD of triplicate determinations of 4 separate experiments. *P < .01 versus response of HUVECs to the various stimuli in the absence of anti–IL-8.
