Production of IL-8 and MCP-1 by HUVECs exposed to iTCC.

(A) Levels of chemokines secreted by HUVECs in the culture supernatant after an overnight incubation with iTCC (5 nM), TNF-α (20 U/mL) or medium alone measured by ELISA. Values are means ± SD of triplicate determinations of 3 separate experiments. *P < .01 versus control. (B) Reverse transcription-PCR of IL-8 and MCP-1 mRNA expression of HUVECs unstimulated or treated with iTCC (5 nM) or TNF-α (200 U/mL).