Effect of iTCC and TNF-α on PMN adhesion to HUVECs.

EC monolayers were pretreated for 4 hours with medium alone or medium containing either iTCC (5 nM) or TNF-α (200 U/mL). PMNs were then added to the monolayer, and the percentage of adherent PMNs was determined after an incubation of 30 minutes at 37°C. Values are mean ± SD of triplicate determinations of 3 separate experiments. *P < .01 versus control.