Fig. 9.
Partial protection from CDDO-Me–induced apoptosis conferred by the U937/S70E Bcl-2 mutant.
Stable transfectants of U937 cells using a serine→alanine Bcl-2 mutant, S70A, and a Ser→Glu mutant, S70E, as well as U937/wt Bcl-2, were exposed to 0.1 μM CDDO-Me for 24 hours. Viability was determined by cell count with trypan blue exclusion, and apoptosis was measured by sub-G1 flow cytometry (acridine orange). S70E, which may mimic a potential phosphate charge, suppressed the CDDO-Me–induced apoptosis.
