Fig. 8.
![Fig. 8. CDDO-Me blockade of Bcl-2 phosphorylation. / U937 cells were radiolabeled with [32P]orthophosphate, treated with 0.1 μM CDDO-Me, and lysed, after which Bcl-2 was immunoprecipitated. Protein was subjected to SDS-PAGE, transferred to a nitrocellulose membrane, and exposed to x-ray film to identify the phosphorylated Bcl-2. The identity of the protein was confirmed by Western blot analysis using the same filter. Pre, pre-immune serum.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/1/10.1182_blood.v99.1.326/6/h80121923008.jpeg?Expires=1722685255&Signature=KHx0U8bknvM3U3sCq1Ud2Rq~Zzvqqi5Csx9tvtzyUxjcUlMVjotxlJQy8iam71vJXtvsMnKUHWRIC2vNP0gIqr4xMaMacA9ZZ87PzmUpABSSBBDeosWUfYOZk2rcXiIFz8GJ11CZmOf1O347zDKLS1kaiKFz9dAjP8LVe4vfwGAJIxGQFWN4crs~gakQ~i~~tm58nVnKg2mOz~wOw8n9xn6XOjaI2MFWu2oea626N14sP2H4wK2z7-FT~BbMHymxmzKxg3z3EyREY9XEzYS0F9sSLI7ujlkpf1ijVDLfCArzJ4s-mWJlAYL0BrRQIo3GW-sFbIMS8Se331HXpFZKpw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
CDDO-Me blockade of Bcl-2 phosphorylation.
U937 cells were radiolabeled with [32P]orthophosphate, treated with 0.1 μM CDDO-Me, and lysed, after which Bcl-2 was immunoprecipitated. Protein was subjected to SDS-PAGE, transferred to a nitrocellulose membrane, and exposed to x-ray film to identify the phosphorylated Bcl-2. The identity of the protein was confirmed by Western blot analysis using the same filter. Pre, pre-immune serum.
