American Society of Hematology

Fig. 6.

$Fig. 6. CDDO-Me–induced Bax expression and translocation to the mitochondria. / (A) Northern blot using Bax probe for HL-60 and U937 cells treated with 1 μM CDDO-Me for 6 hours. GAPDH indicates glyceraldehyde-3-phosphate dehydrogenase (B) NB4 cells were treated with the indicated concentrations of CDDO-Me. Protein lysates were analyzed by Western blot with anti-Bcl-2 and anti-Bax antibodies. (C) Western blot of subcellular fractions to detect Bax in mitochondria heavy membrane (HM) or cytosol (cyt). Tot, total protein. A band detected at 18 kd likely represents a cleavage product of Bax.51$

CDDO-Me–induced Bax expression and translocation to the mitochondria.

(A) Northern blot using Bax probe for HL-60 and U937 cells treated with 1 μM CDDO-Me for 6 hours. GAPDH indicates glyceraldehyde-3-phosphate dehydrogenase (B) NB4 cells were treated with the indicated concentrations of CDDO-Me. Protein lysates were analyzed by Western blot with anti-Bcl-2 and anti-Bax antibodies. (C) Western blot of subcellular fractions to detect Bax in mitochondria heavy membrane (HM) or cytosol (cyt). Tot, total protein. A band detected at 18 kd likely represents a cleavage product of Bax.51

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