Effect of CDDO-Me on leukemic and normal clonogenic progenitors was studied.

(A) CDDO-Me inhibition of AML clonogenic progenitor growth. Data represent average results from 4 different AML and 2 CML-BC samples. Results are expressed as the mean ± SEM of the number of colonies in the presence of increasing concentrations of CDDO-Me (0.05, 0.1, 0.3, 0.5 μM) compared with the number in control cells. The mean number of CFU-blast colonies in the control cultures of experiments 1, 2 3, 4, 5, and 6 were 1268.5 ± 29.0, 350.0 ± 18.4, 473.5 ± 12.0, 360.5 ± 12.0, 65.5 ± 6.4, and 526  ± 14.1, respectively. (B) Effect of CDDO-Me on the growth of normal myeloid and erythroid progenitors using magnetically separated CD34⁺cells. The mean numbers of CFU-GM colonies in the control cultures of experiments 1, 2, and 3 were 687.5 ± 7.5, 995.5 ± 14.5, and 331 ± 7, respectively; the mean numbers of BFU-E colonies were 384.5.5 ± 14.5, 585.5  ± 7.5, and 259.5 ± 7.5.