Flow cytometric analysis reveals an increase in 62L⁺ naı̈ve T-cells in homozygous lck/lck-mExodus-2 transgenic nodes.

Cells were stained with fluorochrome-conjugated mAbs to CD3, CD19, CD4, CD8, and CD62L and then counted by flow cytometry. Analysis of CD3⁺ versus CD19⁺ (A) shows no difference in the relative percent of T cells versus B cells in the spleen, thymus, or lymph nodes. Analysis of CD4⁺, CD8⁺, and CD4⁺/CD8⁺ (B) shows a slight increase (P = .012) in CD4⁺/CD8⁺ T cells in the thymus of the transgenic mice (lck/lck-mCCL21) when compared to normal mice (WT/WT). Analysis of CD3⁺/CD62L⁺versus CD3⁺/CD62L⁻ (C) shows a statistically significant increase in CD62L⁺ T cells in the spleen, thymus, and lymph nodes of lck/lck-mCCL21 transgenic mice, when compared to normal WT mice. Corresponding decreases in CD62L⁻ T-cells in the spleen, thymus, and node are also noted in transgenic mice versus normal mice.