Fig. 5.
Effect of CD30 ligation on the release of CD30, CCL3, and CCL5 by L540 cells.
5 × 104 cells/mL were cultured for 72 hours in 24-well plates coated with 10 μg/mL M67 mAb or BerH2 as a control in the presence or absence of PMA–ion (15 ng/mL PMA + 500 ng/mL ionomycin). CD30, CCL3, and CCL5 concentrations in the culture medium were evaluated by commercially available ELISA. Anti-CD30 agonistic mAb M67 induced increased levels of soluble CD30, CCL3, and CCL5 in the L540 cell culture medium. Data are expressed as mean ± SD of 5 experiments.
