Fig. 4.
Effect of CD30 ligation on CXCL12-induced chemotaxis in the L540 cells.
5 × 105 cells resting or stimulated with PMA–ion (15 ng/mL PMA + 500 ng/mL ionomycin), plastic-bound anti-CD30 agonistic mAb M67 (10 μg/mL), or BerH2 (or anti-CD34 isotype-matched mAb) as a control were subjected to chemotaxis through 8-μm pore Transwell filters to CXCL12 (200 ng/mL) in the lower chamber. Stimulation with M67 increased the sensitivity of L540 cells to the chemotactic effect of CXCL12. The increase in sensitivity was inhibited after 60 minutes of preincubation with neutralizing anti-CXCR4 mAb (12G5, 50 μg/mL). Data are expressed as mean ± SD of 5 experiments.
