Flow cytometric analysis of CXCR4 and CD30 expression in the L540 cells.

1 × 10⁶ cells resting (1) or stimulated for 72 hours with plastic-bound anti-CD30 agonistic mABs M67 (10 μg/mL) (2) or PMA/ion (15 ng/mL PMA + 500 ng/mL ionomycin) (3) were stained with anti-CXCR4-PE and anti-CD30-FITC mAbs and isotype/fluorochrome matched controls. Compared with resting cells (1), M67 (2), but not the nonagonistic anti-CD30 mAb BerH2, induced a clear-cut increase in CXCR4 and a decrease in CD30 MFI, whereas PMA/ion (3) induced a decrease in both CXCR4 and CD30 MFI. The experiment depicted is representative of 10.